Search results for "Aurintricarboxylic acid"

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Aurintricarboxylic acid as a nuclease inhibitor in fungal protoplasts

1986

Aurintricarboxylic acid (ATA), a potent nuclease inhibitor, has been shown to prevent foreign DNA degradation in transformation of Penicillium chrysogenum and Saccharomyces cerevisiae. ATA may be useful not only in vitro but also in vivo.

NucleaseSaccharomyces cerevisiaeBiologyPenicillium chrysogenumbiology.organism_classificationMicrobiologyIn vitrochemistry.chemical_compoundTransformation (genetics)chemistryBiochemistryIn vivoAurintricarboxylic acidGeneticsbiology.proteinMolecular BiologyDNA
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Screening for inhibitors of HIV gp120-CD4 binding using an enzyme-linked immunoabsorbent assay.

1993

Binding of the HIV-1 major viral surface glycoprotein, gp120, to the major cell receptor, CD4, is essential for HIV infection of the target cell and syncytium formation. An enzyme-linked immunoassay using solid phase CD4 was used to quantitate the binding of HIV-1 gp120 to CD4, and to assess the activity and mechanism of action of putative inhibitors of that reaction. Monoclonal antibodies to the gp120 binding site on CD4 (e.g., Leu3a) blocked gp120 binding, while monoclonal antibodies to other portions of CD4 (e.g. OKT4) did not. Both aurintricarboxylic acid and sulfonated polysaccharides (e.g., dextran sulfate) blocked CD4-gp120 interactions by binding to the CD4 component. Human polyclon…

medicine.drug_classvirusesEnzyme-Linked Immunosorbent AssayHIV Envelope Protein gp120Monoclonal antibodyAntiviral Agentschemistry.chemical_compoundPolysaccharidesVirologyLectinsAurintricarboxylic acidmedicineGlycoproteinschemistry.chemical_classificationbiologyLigand binding assayvirus diseasesLectinReproducibility of ResultsMolecular biologyRecombinant ProteinsEnzymechemistryMechanism of actionPolyclonal antibodiesCD4 Antigensbiology.proteinHIV-1medicine.symptomAntibodyJournal of virological methods
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